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KMID : 0545119960060060439
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Journal of Microbiology and Biotechnology
1996 Volume.6 No. 6 p.439 ~ p.444
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Isolation of Chitin-utilizing Bacterium and Production of Its Extracellular Chitinase
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WOO, CHEOL-JOO
YUN, UN-JUNG/PARK, HEUI-DONG
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Abstract
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A bacterial strain, designated as WY22, producing extracellular chitinase was isolated from the soil around the Younduck area, after enrichment culture in a medium containing 1% (w/v) wet colloidal chitin as a sole carbon source. The isolate was identified as a strain of Bacillus sp. based on its morphological and physiological characteristics. It was observed that Bacillus sp. WY22 could inhibit the growth of Fusariun oxysporum with hyphal extention-inhibition assay on potato dextrose agar plate supplemented with 1% collidal chitin. Optimum culture conditions of Bacillus sp. WY22 were examined for chitinase production in a chitin medium. High level production of chitinase was observed not only in the chitin medium but in a medium supplemented with 1% N-glucosamine or lactose instead of chitin. The optimum concentrations of colloidal chitin and yeast extract were 3.0 and 0.5%, and the optimum culture conditions for initial pH of medium and temperature were 7.0 and 30℃, respectively, for the production of chitinase.
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KEYWORD
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